Journal: Nature cardiovascular research
Article Title: A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity.
doi: 10.1038/s44161-022-00183-w
Figure Lengend Snippet: Fig. 6 | EPDCs were found in sub-epicardium and myocardium. a, Spatial validation of this cluster as EPDCs was carried out using a pseudostain targeting strategy from UMAP of scRNA-seq data, with gene expression displayed in RGB color space and a constant blue channel value of 0.2 for heuristic DAPI simulation. b, Staining of fetal hearts using double stains for TM4SF1 and POSTN (bi), PRG4
Article Snippet: The primary antibodies used in this study for immunofluorescent imaging included: UPK3B, PA552696 (Themo Fisher Scientific); MSLN, sc33672 (Santa Cruz Biotechnology); KRT19, sc6278 (Santa Cruz Biotechnology); POSTN, MAB3548 (R&D Systems); DCN, AF143 (R&D Systems); PRG4, MABT400 (Sigma-Aldrich); and TM4SF1, MAB8164 (R&D Systems).
Techniques: Biomarker Discovery, Gene Expression, Staining